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rnascope multiplex fluorescent detection reagents v2 kit  (Advanced Cell Diagnostics Inc)


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    Advanced Cell Diagnostics Inc rnascope multiplex fluorescent detection reagents v2 kit
    Rnascope Multiplex Fluorescent Detection Reagents V2 Kit, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope+multiplex+fluorescent+detection+reagents+v2+kit/rnascope+multiplex+fluorescent+reagent+kit+v2/pm40444991-280-19-48
    Average 90 stars, based on 1 article reviews
    rnascope multiplex fluorescent detection reagents v2 kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    RNAscope:

    Article Title: Revisiting the early event of African swine fever virus DNA replication.
    Article Snippet: .. DNAscope or RNAscope in situ hybridization To detect ASFV DNA (vDNA), in situ hybridization was employed by using the RNAscope Multiplex Fluorescent Detection Reagents v2 Kit (ACD, 323110), and the probes targeting the antisense DNA strand of viral gene B646L, F334L, and H359L were designed and purchased from Advanced Cell Diagnostics (ACD, 320850-I). .. Then, ASFV DNA was detected with the Probe-V-ASFV-MCP-sense-C1 (ACD, 1193281-C1) targeting B646L, Probe-V-ASFV-F334L-sense-C1 (ACD, 1330941-C1) targeting F334L, or Probe-VASFV-H359L-sense-C1 (ACD, 1330951-C1) targeting H359L according to the manufactur er’s protocol.

    Article Title: Synaptic-like transmission between neural axons and arteriolar smooth muscle cells drives cerebral neurovascular coupling
    Article Snippet: Each reaction comprised a total volume of 20 μl containing 2 μl of cDNA, 10 μl of Green Taq Mix (Vazyme, P131-03), each primer pair (Supplementary Table ) at 0.5 μM (Tsingke) and 6 μl of ddH 2 O. .. According to the manufacturer’s protocol, the RNAscope assay was performed using the RNAscope Multiplex Fluorescent Detection Reagents v2 Kit (323110, Advanced Cell Diagnostics). ..

    Article Title: Müller glia cell cycle re-activation by simultaneous cyclin D1 overexpression and p27kip1 knockdown promotes retinal regeneration in mice
    Article Snippet: .. In situ RNA hybridization was performed on retinal sections using the RNAscope Multiplex Fluorescent Detection Reagents V2 kit (Advanced Cell Diagnostics) following the commercial protocols. ..

    Article Title: Synaptic-like transmission between neural axons and arteriolar smooth muscle cells drives cerebral neurovascular coupling.
    Article Snippet: Each reaction comprised a total volume of 20 μl containing 2 μl of cDNA, 10 μl of Green Taq Mix (Vazyme, P131-03), each primer pair (Supplementary Table 1) at 0.5 μM (Tsingke) and 6 μl of ddH2O. .. RNAscope mRNA in situ hybridization assay According to the manufacturer’s protocol, the RNAscope assay was performed using the RNAscope Multiplex Fluorescent Detection Reagents v2 Kit (323110, Advanced Cell Diagnostics). ..

    Article Title: A PTHrP Gradient Drives Mandibular Condylar Chondrogenesis via Runx2.
    Article Snippet: Frozen sections at 12 μm thickness were prepared using a cryostat (Leica CM1860) and adhered on positively charged glass slides (Fisherbrand ColorFrost Plus). .. RNAscope In Situ Hybridization and Immunohistochemistry In situ hybridization was performed with an RNAscope Multiplex Fluorescent Detection Reagents v2 kit (Advanced Cell Diagnostics 323110) using the following probes: Col10a1 (426181), Runx2 (414021), Pth1r (426191), Ihh (413091), and Sp7 (403401). .. After RNAscope, sections were incubated in blocking buffer (3% bovine serum albumin/Tris-buffered saline with Tween (TBST)) for 30 min at room temperature (RT), and subsequently stained with SOX9 polyclonal antibody (1:500; R&D systems, AF3075), RUNX2 polyclonal antibody (1:100; Novus Biological, NBP1-89104), PTHrP polyclonal antibody (1:100; Invitrogen, PA5-57493), PTH1R monoclonal antibody (3D1.1) (1:200; Novus Biological, NBP1-40067), DsRed Polyclonal Antibody (Living Colors) (1:700; Takara Bio, 632496), or mCherry monoclonal antibody (16D7) (1:400; Invitrogen, M11217), overnight at 4°C.

    Article Title: Orexinergic projections to substantia innominata mediate arousal and analgesia
    Article Snippet: .. The staining was performed with the RNAscope Multiplex Fluorescent Detection Reagents v2 kit (323110, Advanced Cell Diagnostics) according to the user manual. .. Probes for OX1R (Hcrtr1, Cat No. 466631-C1), ChAT (CHAT, Cat No. 408731-C1), vGlut2 (Slc17a6, Cat No. 428871-C2), vGAT (Slc32a1, Cat No. 319191-C2), TH (TH, Cat No. 317621-C2), and OX2R (Hcrtr2, Cat No. 581631-C3) were commercially available by Advanced Cell Diagnostics.

    Article Title: Hedgehog activation promotes osteogenic fates of growth plate resting zone chondrocytes through transient clonal competency
    Article Snippet: Stained samples were mounted in TBS with No.1.5 coverslips (Thermo Fisher Scientific). .. In situ hybridization was performed with RNAscope Multiplex Fluorescent Detection Reagents v2 kit (Advanced Cell Diagnostics, catalog 323110) using the following riboprobes: Ptch1 exon8–9 (specific to exons 8 and 9 flanked by loxP sites, catalog 476231) and Smo (catalog 318411). ..

    Article Title: Müller glia cell cycle re-activation by simultaneous cyclin D1 overexpression and p27 kip1 knockdown promotes retinal regeneration in mice
    Article Snippet: .. In situ RNA hybridization was performed using the RNAscope Multiplex Fluorescent Detection Reagents V2 kit (Advanced Cell Diagnostics) following the commercial protocols. ..

    In Situ Hybridization:

    Article Title: Revisiting the early event of African swine fever virus DNA replication.
    Article Snippet: .. DNAscope or RNAscope in situ hybridization To detect ASFV DNA (vDNA), in situ hybridization was employed by using the RNAscope Multiplex Fluorescent Detection Reagents v2 Kit (ACD, 323110), and the probes targeting the antisense DNA strand of viral gene B646L, F334L, and H359L were designed and purchased from Advanced Cell Diagnostics (ACD, 320850-I). .. Then, ASFV DNA was detected with the Probe-V-ASFV-MCP-sense-C1 (ACD, 1193281-C1) targeting B646L, Probe-V-ASFV-F334L-sense-C1 (ACD, 1330941-C1) targeting F334L, or Probe-VASFV-H359L-sense-C1 (ACD, 1330951-C1) targeting H359L according to the manufactur er’s protocol.

    Article Title: Synaptic-like transmission between neural axons and arteriolar smooth muscle cells drives cerebral neurovascular coupling.
    Article Snippet: Each reaction comprised a total volume of 20 μl containing 2 μl of cDNA, 10 μl of Green Taq Mix (Vazyme, P131-03), each primer pair (Supplementary Table 1) at 0.5 μM (Tsingke) and 6 μl of ddH2O. .. RNAscope mRNA in situ hybridization assay According to the manufacturer’s protocol, the RNAscope assay was performed using the RNAscope Multiplex Fluorescent Detection Reagents v2 Kit (323110, Advanced Cell Diagnostics). ..

    Article Title: A PTHrP Gradient Drives Mandibular Condylar Chondrogenesis via Runx2.
    Article Snippet: Frozen sections at 12 μm thickness were prepared using a cryostat (Leica CM1860) and adhered on positively charged glass slides (Fisherbrand ColorFrost Plus). .. RNAscope In Situ Hybridization and Immunohistochemistry In situ hybridization was performed with an RNAscope Multiplex Fluorescent Detection Reagents v2 kit (Advanced Cell Diagnostics 323110) using the following probes: Col10a1 (426181), Runx2 (414021), Pth1r (426191), Ihh (413091), and Sp7 (403401). .. After RNAscope, sections were incubated in blocking buffer (3% bovine serum albumin/Tris-buffered saline with Tween (TBST)) for 30 min at room temperature (RT), and subsequently stained with SOX9 polyclonal antibody (1:500; R&D systems, AF3075), RUNX2 polyclonal antibody (1:100; Novus Biological, NBP1-89104), PTHrP polyclonal antibody (1:100; Invitrogen, PA5-57493), PTH1R monoclonal antibody (3D1.1) (1:200; Novus Biological, NBP1-40067), DsRed Polyclonal Antibody (Living Colors) (1:700; Takara Bio, 632496), or mCherry monoclonal antibody (16D7) (1:400; Invitrogen, M11217), overnight at 4°C.

    Article Title: Hedgehog activation promotes osteogenic fates of growth plate resting zone chondrocytes through transient clonal competency
    Article Snippet: Stained samples were mounted in TBS with No.1.5 coverslips (Thermo Fisher Scientific). .. In situ hybridization was performed with RNAscope Multiplex Fluorescent Detection Reagents v2 kit (Advanced Cell Diagnostics, catalog 323110) using the following riboprobes: Ptch1 exon8–9 (specific to exons 8 and 9 flanked by loxP sites, catalog 476231) and Smo (catalog 318411). ..

    Multiplex Assay:

    Article Title: Revisiting the early event of African swine fever virus DNA replication.
    Article Snippet: .. DNAscope or RNAscope in situ hybridization To detect ASFV DNA (vDNA), in situ hybridization was employed by using the RNAscope Multiplex Fluorescent Detection Reagents v2 Kit (ACD, 323110), and the probes targeting the antisense DNA strand of viral gene B646L, F334L, and H359L were designed and purchased from Advanced Cell Diagnostics (ACD, 320850-I). .. Then, ASFV DNA was detected with the Probe-V-ASFV-MCP-sense-C1 (ACD, 1193281-C1) targeting B646L, Probe-V-ASFV-F334L-sense-C1 (ACD, 1330941-C1) targeting F334L, or Probe-VASFV-H359L-sense-C1 (ACD, 1330951-C1) targeting H359L according to the manufactur er’s protocol.

    Article Title: Synaptic-like transmission between neural axons and arteriolar smooth muscle cells drives cerebral neurovascular coupling
    Article Snippet: Each reaction comprised a total volume of 20 μl containing 2 μl of cDNA, 10 μl of Green Taq Mix (Vazyme, P131-03), each primer pair (Supplementary Table ) at 0.5 μM (Tsingke) and 6 μl of ddH 2 O. .. According to the manufacturer’s protocol, the RNAscope assay was performed using the RNAscope Multiplex Fluorescent Detection Reagents v2 Kit (323110, Advanced Cell Diagnostics). ..

    Article Title: Müller glia cell cycle re-activation by simultaneous cyclin D1 overexpression and p27kip1 knockdown promotes retinal regeneration in mice
    Article Snippet: .. In situ RNA hybridization was performed on retinal sections using the RNAscope Multiplex Fluorescent Detection Reagents V2 kit (Advanced Cell Diagnostics) following the commercial protocols. ..

    Article Title: Synaptic-like transmission between neural axons and arteriolar smooth muscle cells drives cerebral neurovascular coupling.
    Article Snippet: Each reaction comprised a total volume of 20 μl containing 2 μl of cDNA, 10 μl of Green Taq Mix (Vazyme, P131-03), each primer pair (Supplementary Table 1) at 0.5 μM (Tsingke) and 6 μl of ddH2O. .. RNAscope mRNA in situ hybridization assay According to the manufacturer’s protocol, the RNAscope assay was performed using the RNAscope Multiplex Fluorescent Detection Reagents v2 Kit (323110, Advanced Cell Diagnostics). ..

    Article Title: A PTHrP Gradient Drives Mandibular Condylar Chondrogenesis via Runx2.
    Article Snippet: Frozen sections at 12 μm thickness were prepared using a cryostat (Leica CM1860) and adhered on positively charged glass slides (Fisherbrand ColorFrost Plus). .. RNAscope In Situ Hybridization and Immunohistochemistry In situ hybridization was performed with an RNAscope Multiplex Fluorescent Detection Reagents v2 kit (Advanced Cell Diagnostics 323110) using the following probes: Col10a1 (426181), Runx2 (414021), Pth1r (426191), Ihh (413091), and Sp7 (403401). .. After RNAscope, sections were incubated in blocking buffer (3% bovine serum albumin/Tris-buffered saline with Tween (TBST)) for 30 min at room temperature (RT), and subsequently stained with SOX9 polyclonal antibody (1:500; R&D systems, AF3075), RUNX2 polyclonal antibody (1:100; Novus Biological, NBP1-89104), PTHrP polyclonal antibody (1:100; Invitrogen, PA5-57493), PTH1R monoclonal antibody (3D1.1) (1:200; Novus Biological, NBP1-40067), DsRed Polyclonal Antibody (Living Colors) (1:700; Takara Bio, 632496), or mCherry monoclonal antibody (16D7) (1:400; Invitrogen, M11217), overnight at 4°C.

    Article Title: Orexinergic projections to substantia innominata mediate arousal and analgesia
    Article Snippet: .. The staining was performed with the RNAscope Multiplex Fluorescent Detection Reagents v2 kit (323110, Advanced Cell Diagnostics) according to the user manual. .. Probes for OX1R (Hcrtr1, Cat No. 466631-C1), ChAT (CHAT, Cat No. 408731-C1), vGlut2 (Slc17a6, Cat No. 428871-C2), vGAT (Slc32a1, Cat No. 319191-C2), TH (TH, Cat No. 317621-C2), and OX2R (Hcrtr2, Cat No. 581631-C3) were commercially available by Advanced Cell Diagnostics.

    Article Title: Hedgehog activation promotes osteogenic fates of growth plate resting zone chondrocytes through transient clonal competency
    Article Snippet: Stained samples were mounted in TBS with No.1.5 coverslips (Thermo Fisher Scientific). .. In situ hybridization was performed with RNAscope Multiplex Fluorescent Detection Reagents v2 kit (Advanced Cell Diagnostics, catalog 323110) using the following riboprobes: Ptch1 exon8–9 (specific to exons 8 and 9 flanked by loxP sites, catalog 476231) and Smo (catalog 318411). ..

    Article Title: Müller glia cell cycle re-activation by simultaneous cyclin D1 overexpression and p27 kip1 knockdown promotes retinal regeneration in mice
    Article Snippet: .. In situ RNA hybridization was performed using the RNAscope Multiplex Fluorescent Detection Reagents V2 kit (Advanced Cell Diagnostics) following the commercial protocols. ..

    In Situ:

    Article Title: Müller glia cell cycle re-activation by simultaneous cyclin D1 overexpression and p27kip1 knockdown promotes retinal regeneration in mice
    Article Snippet: .. In situ RNA hybridization was performed on retinal sections using the RNAscope Multiplex Fluorescent Detection Reagents V2 kit (Advanced Cell Diagnostics) following the commercial protocols. ..

    Article Title: Müller glia cell cycle re-activation by simultaneous cyclin D1 overexpression and p27 kip1 knockdown promotes retinal regeneration in mice
    Article Snippet: .. In situ RNA hybridization was performed using the RNAscope Multiplex Fluorescent Detection Reagents V2 kit (Advanced Cell Diagnostics) following the commercial protocols. ..

    Hybridization:

    Article Title: Müller glia cell cycle re-activation by simultaneous cyclin D1 overexpression and p27kip1 knockdown promotes retinal regeneration in mice
    Article Snippet: .. In situ RNA hybridization was performed on retinal sections using the RNAscope Multiplex Fluorescent Detection Reagents V2 kit (Advanced Cell Diagnostics) following the commercial protocols. ..

    Article Title: Müller glia cell cycle re-activation by simultaneous cyclin D1 overexpression and p27 kip1 knockdown promotes retinal regeneration in mice
    Article Snippet: .. In situ RNA hybridization was performed using the RNAscope Multiplex Fluorescent Detection Reagents V2 kit (Advanced Cell Diagnostics) following the commercial protocols. ..

    Immunohistochemistry:

    Article Title: A PTHrP Gradient Drives Mandibular Condylar Chondrogenesis via Runx2.
    Article Snippet: Frozen sections at 12 μm thickness were prepared using a cryostat (Leica CM1860) and adhered on positively charged glass slides (Fisherbrand ColorFrost Plus). .. RNAscope In Situ Hybridization and Immunohistochemistry In situ hybridization was performed with an RNAscope Multiplex Fluorescent Detection Reagents v2 kit (Advanced Cell Diagnostics 323110) using the following probes: Col10a1 (426181), Runx2 (414021), Pth1r (426191), Ihh (413091), and Sp7 (403401). .. After RNAscope, sections were incubated in blocking buffer (3% bovine serum albumin/Tris-buffered saline with Tween (TBST)) for 30 min at room temperature (RT), and subsequently stained with SOX9 polyclonal antibody (1:500; R&D systems, AF3075), RUNX2 polyclonal antibody (1:100; Novus Biological, NBP1-89104), PTHrP polyclonal antibody (1:100; Invitrogen, PA5-57493), PTH1R monoclonal antibody (3D1.1) (1:200; Novus Biological, NBP1-40067), DsRed Polyclonal Antibody (Living Colors) (1:700; Takara Bio, 632496), or mCherry monoclonal antibody (16D7) (1:400; Invitrogen, M11217), overnight at 4°C.

    Staining:

    Article Title: Orexinergic projections to substantia innominata mediate arousal and analgesia
    Article Snippet: .. The staining was performed with the RNAscope Multiplex Fluorescent Detection Reagents v2 kit (323110, Advanced Cell Diagnostics) according to the user manual. .. Probes for OX1R (Hcrtr1, Cat No. 466631-C1), ChAT (CHAT, Cat No. 408731-C1), vGlut2 (Slc17a6, Cat No. 428871-C2), vGAT (Slc32a1, Cat No. 319191-C2), TH (TH, Cat No. 317621-C2), and OX2R (Hcrtr2, Cat No. 581631-C3) were commercially available by Advanced Cell Diagnostics.



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    (A-E) Dual immunofluorescence and <t>RNAscope</t> staining for FSP1 protein (red), Axin2 mRNA (yellow), Lgr5 mRNA (cyan), and DAPI (grey) between E16.5 and P28 in CD1 mice (n=3). (A) Dash line indicates condyle surface. (F) Quantification of FSP1-positive cells and Axin2 -positive cells during condyle growth. Error bar = ±1S.D.; n=3; two-way ANOVA flowed by Tukey’s multiple comparisons test (*P<0.05, **P<0.01, ****P<0.0001). (G) Tamoxifen was administered to Axin2-CreERT2;tdTom mice at P16 and collected at P18 (n=3). (H) Immunofluorescence staining for FSP1 (green), RFP (red), and DAPI (blue). (I) The box shows a zoomed-in image; arrows indicate FSP1-positive cells (green) co-expressing with Axin2 (red). (J-M) Dual immunofluorescence and RNAscope for FSP1 (red), Gli1 mRNA (yellow), Scx mRNA (cyan), and DAPI (grey) in different stages of CD1 mice (n=3). (N) BrdU was administered to CD1 mice at E17.5, E18.5 (n=3). BrdU-positive label retaining cells (LRCs) were chased in P2, P21, and P48. (O-Q) Immunofluorescence staining for BrdU (green), FSP1 (red), and DAPI (blue). (P,Q) Arrows indicate FSP1-positive cells (red) co-stained with BrdU LRCs (green). Scale bar in A-E and I-Q: 20 µm, scale bar in H: 100 µm. ANOVA, analysis of variance; BrdU, 5-Bromo-2’-deoxyuridine; RFP, red <t>fluorescent</t> protein; Tmx, tamoxifen; Scx, scleraxis.
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    (A-E) Dual immunofluorescence and <t>RNAscope</t> staining for FSP1 protein (red), Axin2 mRNA (yellow), Lgr5 mRNA (cyan), and DAPI (grey) between E16.5 and P28 in CD1 mice (n=3). (A) Dash line indicates condyle surface. (F) Quantification of FSP1-positive cells and Axin2 -positive cells during condyle growth. Error bar = ±1S.D.; n=3; two-way ANOVA flowed by Tukey’s multiple comparisons test (*P<0.05, **P<0.01, ****P<0.0001). (G) Tamoxifen was administered to Axin2-CreERT2;tdTom mice at P16 and collected at P18 (n=3). (H) Immunofluorescence staining for FSP1 (green), RFP (red), and DAPI (blue). (I) The box shows a zoomed-in image; arrows indicate FSP1-positive cells (green) co-expressing with Axin2 (red). (J-M) Dual immunofluorescence and RNAscope for FSP1 (red), Gli1 mRNA (yellow), Scx mRNA (cyan), and DAPI (grey) in different stages of CD1 mice (n=3). (N) BrdU was administered to CD1 mice at E17.5, E18.5 (n=3). BrdU-positive label retaining cells (LRCs) were chased in P2, P21, and P48. (O-Q) Immunofluorescence staining for BrdU (green), FSP1 (red), and DAPI (blue). (P,Q) Arrows indicate FSP1-positive cells (red) co-stained with BrdU LRCs (green). Scale bar in A-E and I-Q: 20 µm, scale bar in H: 100 µm. ANOVA, analysis of variance; BrdU, 5-Bromo-2’-deoxyuridine; RFP, red <t>fluorescent</t> protein; Tmx, tamoxifen; Scx, scleraxis.
    Rnascope Multiplex Fluorescent Detection Reagent V2 Kit, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A-E) Dual immunofluorescence and RNAscope staining for FSP1 protein (red), Axin2 mRNA (yellow), Lgr5 mRNA (cyan), and DAPI (grey) between E16.5 and P28 in CD1 mice (n=3). (A) Dash line indicates condyle surface. (F) Quantification of FSP1-positive cells and Axin2 -positive cells during condyle growth. Error bar = ±1S.D.; n=3; two-way ANOVA flowed by Tukey’s multiple comparisons test (*P<0.05, **P<0.01, ****P<0.0001). (G) Tamoxifen was administered to Axin2-CreERT2;tdTom mice at P16 and collected at P18 (n=3). (H) Immunofluorescence staining for FSP1 (green), RFP (red), and DAPI (blue). (I) The box shows a zoomed-in image; arrows indicate FSP1-positive cells (green) co-expressing with Axin2 (red). (J-M) Dual immunofluorescence and RNAscope for FSP1 (red), Gli1 mRNA (yellow), Scx mRNA (cyan), and DAPI (grey) in different stages of CD1 mice (n=3). (N) BrdU was administered to CD1 mice at E17.5, E18.5 (n=3). BrdU-positive label retaining cells (LRCs) were chased in P2, P21, and P48. (O-Q) Immunofluorescence staining for BrdU (green), FSP1 (red), and DAPI (blue). (P,Q) Arrows indicate FSP1-positive cells (red) co-stained with BrdU LRCs (green). Scale bar in A-E and I-Q: 20 µm, scale bar in H: 100 µm. ANOVA, analysis of variance; BrdU, 5-Bromo-2’-deoxyuridine; RFP, red fluorescent protein; Tmx, tamoxifen; Scx, scleraxis.

    Journal: bioRxiv

    Article Title: FSP1 stem/progenitor cells are essential for TMJ growth and homeostasis

    doi: 10.1101/2024.08.16.608236

    Figure Lengend Snippet: (A-E) Dual immunofluorescence and RNAscope staining for FSP1 protein (red), Axin2 mRNA (yellow), Lgr5 mRNA (cyan), and DAPI (grey) between E16.5 and P28 in CD1 mice (n=3). (A) Dash line indicates condyle surface. (F) Quantification of FSP1-positive cells and Axin2 -positive cells during condyle growth. Error bar = ±1S.D.; n=3; two-way ANOVA flowed by Tukey’s multiple comparisons test (*P<0.05, **P<0.01, ****P<0.0001). (G) Tamoxifen was administered to Axin2-CreERT2;tdTom mice at P16 and collected at P18 (n=3). (H) Immunofluorescence staining for FSP1 (green), RFP (red), and DAPI (blue). (I) The box shows a zoomed-in image; arrows indicate FSP1-positive cells (green) co-expressing with Axin2 (red). (J-M) Dual immunofluorescence and RNAscope for FSP1 (red), Gli1 mRNA (yellow), Scx mRNA (cyan), and DAPI (grey) in different stages of CD1 mice (n=3). (N) BrdU was administered to CD1 mice at E17.5, E18.5 (n=3). BrdU-positive label retaining cells (LRCs) were chased in P2, P21, and P48. (O-Q) Immunofluorescence staining for BrdU (green), FSP1 (red), and DAPI (blue). (P,Q) Arrows indicate FSP1-positive cells (red) co-stained with BrdU LRCs (green). Scale bar in A-E and I-Q: 20 µm, scale bar in H: 100 µm. ANOVA, analysis of variance; BrdU, 5-Bromo-2’-deoxyuridine; RFP, red fluorescent protein; Tmx, tamoxifen; Scx, scleraxis.

    Article Snippet: RNAscope from Advanced Cell Diagnostics (RNAscope® Multiplex Fluorescent Detection Reagents Kit v2) was used following the manufacturer’s instructions.

    Techniques: Immunofluorescence, RNAscope, Staining, Expressing

    (A-F) H&E staining and immunofluorescence staining in 12-week-old FSP1-Cre;βcatGOF (n=3) and Cre-negative βcatGOF littermate controls. (A,D) H&E staining shows condyle morphology. (B,E) The box shows a zoomed-in image of superficial layer of the condyle. (C,F) Immunofluorescence staining for FSP1 (red), SOX9 (green), COL2A1 (grey), and DAPI (blue). (G,H) Representative images of immunofluorescence with RNAscope staining for FSP1 (red), Axin2 (green), and DAPI (blue) in 12-week-old FSP1-Cre;βcatGOF and Cre-negative βcatGOF littermate controls. (I) FSP1-positive cells and Axin2 -positive cells were quantified using Qupath software. Error bar = ±S.D.; n=3; two-way ANOVA followed by Sidak multiple comparisons test (***P<0.001, ****P<0.0001). Scale bar B-C, E-F, G-H: 20 µm, scale bar A and D: 100 µm. βcat , β-catenin; GOF, gain of function.

    Journal: bioRxiv

    Article Title: FSP1 stem/progenitor cells are essential for TMJ growth and homeostasis

    doi: 10.1101/2024.08.16.608236

    Figure Lengend Snippet: (A-F) H&E staining and immunofluorescence staining in 12-week-old FSP1-Cre;βcatGOF (n=3) and Cre-negative βcatGOF littermate controls. (A,D) H&E staining shows condyle morphology. (B,E) The box shows a zoomed-in image of superficial layer of the condyle. (C,F) Immunofluorescence staining for FSP1 (red), SOX9 (green), COL2A1 (grey), and DAPI (blue). (G,H) Representative images of immunofluorescence with RNAscope staining for FSP1 (red), Axin2 (green), and DAPI (blue) in 12-week-old FSP1-Cre;βcatGOF and Cre-negative βcatGOF littermate controls. (I) FSP1-positive cells and Axin2 -positive cells were quantified using Qupath software. Error bar = ±S.D.; n=3; two-way ANOVA followed by Sidak multiple comparisons test (***P<0.001, ****P<0.0001). Scale bar B-C, E-F, G-H: 20 µm, scale bar A and D: 100 µm. βcat , β-catenin; GOF, gain of function.

    Article Snippet: RNAscope from Advanced Cell Diagnostics (RNAscope® Multiplex Fluorescent Detection Reagents Kit v2) was used following the manufacturer’s instructions.

    Techniques: Staining, Immunofluorescence, RNAscope, Software